anti zikv ns1 antibody Search Results


zikv ns1  (Native Antigen Inc)
94
Native Antigen Inc zikv ns1
Isotype specificity of ZIKV‐immune sera. ELISA plates were coated with ZIKV <t>NS1</t> (a, c) or ZIKV VLP (b, d), and a 1:100 dilution ( n = 28) (a, b) or serial dilutions ( n = 11) (c, d) of ZIKV‐immune sera were added to plates. Following incubation and washes, optimized concentrations of polyclonal secondary IgG, and monoclonal Abs against the hinge or Fc portion of IgG1, IgG2, IgG3 and IgG4 Abs were added to wells (a) and (b) and IgG1 hinge Abs to (c) and (d). OD values shown at 450 nm. OD values to ZIKV VLP and ZIKV NS1 using a 1:100 dilution of ZIKV‐naïve sera ( n = 4), <0·2 with polyclonal secondary IgG and <0·1 with IgG1 hinge, IgG1 Fc, IgG2, IgG3 and IgG4 secondary Abs. Bars represent mean with standard error of the mean of the OD values. * P < 0·05, *** P < 0·001 and **** P < 0·0001 were obtained using the non‐parametric Friedman test (three or more matched groups) with Dunn's multiple comparisons test
Zikv Ns1, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+zikv+ns1+antibody/Zika+Virus+NS1+Protein+(Uganda+Strain)/pmc08442231-137-14-19
Average 94 stars, based on 1 article reviews
zikv ns1 - by Bioz Stars, 2026-11
94/100 stars
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anti-zikv ns1 monoclonal antibody  (BioNano Genomics)
90
BioNano Genomics anti-zikv ns1 monoclonal antibody
Operation of the MOnITOR chip for one-step indirect immunoassays. (a) In the ‘Preparation’ stage, the chip immediately started to operate. (b) Engineered bead lines were secured on the reaction zone. The scale bar represents 100 μm. (c) Red-dyed sample was loaded. Trapped gas bubbles eventually diffused through the PDMS matrix (blue arrows). Wasted volumes in the loading and dummy channels consume only 1.6% of the loaded sample. (d,e) Sequentially-loaded blue-dyed buffer flowed and filled the storage chamber. During storage chamber filling, 2nd Ab was rehydrated. Rehydrated 2nd Ab diffusion toward the buffer inlet was prevented by the delaying channel. (f) When loading sample with anti-ZIKV NS1 and anti-CHIKV <t>E2</t> <t>mAb,</t> only the corresponding beads reacted. (g) Buffer continuously flowed toward the reaction zone by contouring the empty sample inlet. (h) Unbound 2nd Ab were washed by flowing buffer. An image of the reaction zone was acquired in this stage. The scale bar represents 1 cm. (i) In the case of (f), the transferred 2nd Ab only reacted with ZIKV, CHIKV, and + beads. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Anti Zikv Ns1 Monoclonal Antibody, supplied by BioNano Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+zikv+ns1+antibody/anti+zikv+ns1+monoclonal+antibody/pmc08166042-55-7-18
Average 90 stars, based on 1 article reviews
anti-zikv ns1 monoclonal antibody - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
anti-zika virus(zikv)(strain zika sph2015) zikv-ns1 monoclonal antibody  (Elabscience Biotechnology)
N/A
Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replicaiton. The possible effects of NS1 on hosts include: localization to host cell surface and secreted extracellularly, modelates signalling of
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anti-zika virus (zikv) (strain zika sph2015) zikv-ns1 antibody  (TargetMol)
N/A
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anti-zika virus (zikv) (strain zika sph2015) zikv-ns1 antibody-hrp  (TargetMol)
N/A
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Image Search Results


Isotype specificity of ZIKV‐immune sera. ELISA plates were coated with ZIKV NS1 (a, c) or ZIKV VLP (b, d), and a 1:100 dilution ( n = 28) (a, b) or serial dilutions ( n = 11) (c, d) of ZIKV‐immune sera were added to plates. Following incubation and washes, optimized concentrations of polyclonal secondary IgG, and monoclonal Abs against the hinge or Fc portion of IgG1, IgG2, IgG3 and IgG4 Abs were added to wells (a) and (b) and IgG1 hinge Abs to (c) and (d). OD values shown at 450 nm. OD values to ZIKV VLP and ZIKV NS1 using a 1:100 dilution of ZIKV‐naïve sera ( n = 4), <0·2 with polyclonal secondary IgG and <0·1 with IgG1 hinge, IgG1 Fc, IgG2, IgG3 and IgG4 secondary Abs. Bars represent mean with standard error of the mean of the OD values. * P < 0·05, *** P < 0·001 and **** P < 0·0001 were obtained using the non‐parametric Friedman test (three or more matched groups) with Dunn's multiple comparisons test

Journal: Immunology

Article Title: Non‐structural protein 1‐specific antibodies directed against Zika virus in humans mediate antibody‐dependent cellular cytotoxicity

doi: 10.1111/imm.13380

Figure Lengend Snippet: Isotype specificity of ZIKV‐immune sera. ELISA plates were coated with ZIKV NS1 (a, c) or ZIKV VLP (b, d), and a 1:100 dilution ( n = 28) (a, b) or serial dilutions ( n = 11) (c, d) of ZIKV‐immune sera were added to plates. Following incubation and washes, optimized concentrations of polyclonal secondary IgG, and monoclonal Abs against the hinge or Fc portion of IgG1, IgG2, IgG3 and IgG4 Abs were added to wells (a) and (b) and IgG1 hinge Abs to (c) and (d). OD values shown at 450 nm. OD values to ZIKV VLP and ZIKV NS1 using a 1:100 dilution of ZIKV‐naïve sera ( n = 4), <0·2 with polyclonal secondary IgG and <0·1 with IgG1 hinge, IgG1 Fc, IgG2, IgG3 and IgG4 secondary Abs. Bars represent mean with standard error of the mean of the OD values. * P < 0·05, *** P < 0·001 and **** P < 0·0001 were obtained using the non‐parametric Friedman test (three or more matched groups) with Dunn's multiple comparisons test

Article Snippet: We generated CEM‐NK R cells stably expressing ZIKV NS1 and established assays using a ZIKV NS1‐specific human IgG1 mAb (Native Antigen Company).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation

Frequencies of ZIKV‐specific IgG and IgG1 B‐cell responses using fluorescently labelled ZIKV or ZIKV NS1. PBMCs from ZIKV‐immune and ZIKV‐naïve donors were stimulated in vitro for seven days with r848 + rIL‐2. FluoroSpot plates were coated with IgG and IgG1 capture Abs. (a) Images of wells containing media, total IgG, ZIKV and ZIKV NS1 IgG‐ or IgG1‐specific MBCs from one representative donor. (b) Frequencies of ZIKV (green) and NS1‐specific (red) ASCs per 10 6 input cells in MBC cultures from 12 ZIKV‐immune and three ZIKV‐naïve donors. Values are the mean of duplicate wells for each condition. The supernatants were evaluated for isotype‐specific Abs to (c) ZIKV VLPs and (D) ZIKV NS1. * P < 0·05, ** P < 0·01 and **** P < 0·0001 were obtained using the non‐parametric Mann‐Whitney or Friedman test (three or more matched groups) with Dunn's multiple comparisons test

Journal: Immunology

Article Title: Non‐structural protein 1‐specific antibodies directed against Zika virus in humans mediate antibody‐dependent cellular cytotoxicity

doi: 10.1111/imm.13380

Figure Lengend Snippet: Frequencies of ZIKV‐specific IgG and IgG1 B‐cell responses using fluorescently labelled ZIKV or ZIKV NS1. PBMCs from ZIKV‐immune and ZIKV‐naïve donors were stimulated in vitro for seven days with r848 + rIL‐2. FluoroSpot plates were coated with IgG and IgG1 capture Abs. (a) Images of wells containing media, total IgG, ZIKV and ZIKV NS1 IgG‐ or IgG1‐specific MBCs from one representative donor. (b) Frequencies of ZIKV (green) and NS1‐specific (red) ASCs per 10 6 input cells in MBC cultures from 12 ZIKV‐immune and three ZIKV‐naïve donors. Values are the mean of duplicate wells for each condition. The supernatants were evaluated for isotype‐specific Abs to (c) ZIKV VLPs and (D) ZIKV NS1. * P < 0·05, ** P < 0·01 and **** P < 0·0001 were obtained using the non‐parametric Mann‐Whitney or Friedman test (three or more matched groups) with Dunn's multiple comparisons test

Article Snippet: We generated CEM‐NK R cells stably expressing ZIKV NS1 and established assays using a ZIKV NS1‐specific human IgG1 mAb (Native Antigen Company).

Techniques: In Vitro, MANN-WHITNEY

Opsonization and ADCC activity of a ZIKV NS1 monoclonal Ab. (a) K562 cells transfected with DC‐SIGN were infected with ZIKV (moi = 0·1 and 1), and 24 h later, the expression of E and NS1 was measured with monoclonal Abs 4G2 and B4 respectively. (b) Opsonization of ZIKV NS1‐transfected CEM‐NK R cells with a mAb to ZIKV NS1 from Native Antigen Company. CEM‐NK R cells transfected with ZIKV NS1 (c and d) or control CEM‐NK R cells (e and f) were opsonized with a ZIKV NS1 mAb (d and f), added to PBMC. A standard lymphocyte gate based upon light scatter properties followed by selection of singlet viable cells and dump channel exclusion (CD3 − T cells) defined the non‐T‐cell lymphocyte population, and Abs to CD16 and CD56 identified NK cells

Journal: Immunology

Article Title: Non‐structural protein 1‐specific antibodies directed against Zika virus in humans mediate antibody‐dependent cellular cytotoxicity

doi: 10.1111/imm.13380

Figure Lengend Snippet: Opsonization and ADCC activity of a ZIKV NS1 monoclonal Ab. (a) K562 cells transfected with DC‐SIGN were infected with ZIKV (moi = 0·1 and 1), and 24 h later, the expression of E and NS1 was measured with monoclonal Abs 4G2 and B4 respectively. (b) Opsonization of ZIKV NS1‐transfected CEM‐NK R cells with a mAb to ZIKV NS1 from Native Antigen Company. CEM‐NK R cells transfected with ZIKV NS1 (c and d) or control CEM‐NK R cells (e and f) were opsonized with a ZIKV NS1 mAb (d and f), added to PBMC. A standard lymphocyte gate based upon light scatter properties followed by selection of singlet viable cells and dump channel exclusion (CD3 − T cells) defined the non‐T‐cell lymphocyte population, and Abs to CD16 and CD56 identified NK cells

Article Snippet: We generated CEM‐NK R cells stably expressing ZIKV NS1 and established assays using a ZIKV NS1‐specific human IgG1 mAb (Native Antigen Company).

Techniques: Activity Assay, Transfection, Infection, Expressing, Selection

NK cell activation and degranulation in the presence of ZIKV‐immune sera. 1:100 dilution and 1:1600 dilution of polyclonal sera from ZIKV‐immune individuals were evaluated for their ability to mediate ADCC of ZIKV NS1 CEM‐NK R cells using intracellular cytokine assays. Shown are the frequencies of NK cells that (a) secrete IFN‐γ, (B) express CD107 or (C) express both CD107 and IFN‐γ in the presence of ZIKV‐immune sera. Background levels were subtracted

Journal: Immunology

Article Title: Non‐structural protein 1‐specific antibodies directed against Zika virus in humans mediate antibody‐dependent cellular cytotoxicity

doi: 10.1111/imm.13380

Figure Lengend Snippet: NK cell activation and degranulation in the presence of ZIKV‐immune sera. 1:100 dilution and 1:1600 dilution of polyclonal sera from ZIKV‐immune individuals were evaluated for their ability to mediate ADCC of ZIKV NS1 CEM‐NK R cells using intracellular cytokine assays. Shown are the frequencies of NK cells that (a) secrete IFN‐γ, (B) express CD107 or (C) express both CD107 and IFN‐γ in the presence of ZIKV‐immune sera. Background levels were subtracted

Article Snippet: We generated CEM‐NK R cells stably expressing ZIKV NS1 and established assays using a ZIKV NS1‐specific human IgG1 mAb (Native Antigen Company).

Techniques: Activation Assay

Opsonization and target cell lysis using an NK‐TVA image cytometry assay. (a) Opsonization of ZIKV NS1 CEM‐NK R cells by ZIKV convalescent sera. (b) Representative images of wells acquired by an ImmunoSpot S5 Analyser of an NK‐TVA assay where ethanol, ZIKV NS1 or IgG Ab was added to the wells containing effector and target cells. (c) Using an optimized number of target cells, effector PBMC as a source of NK cells and different dilutions of naïve sera ( n = 5), positive control sera that opsonized ZIKV NS1 CEM‐NK R targets ( n = 5) and experimental sera ( n = 12), the loss of target cells at the single‐cell level was measured by the NK‐TVA image cytometry assay and calculated as a percentage of lysed target cells. * P < 0·05 and ** P < 0·01 were obtained using a Kruskal–Wallis test with Dunn's multiple comparisons test

Journal: Immunology

Article Title: Non‐structural protein 1‐specific antibodies directed against Zika virus in humans mediate antibody‐dependent cellular cytotoxicity

doi: 10.1111/imm.13380

Figure Lengend Snippet: Opsonization and target cell lysis using an NK‐TVA image cytometry assay. (a) Opsonization of ZIKV NS1 CEM‐NK R cells by ZIKV convalescent sera. (b) Representative images of wells acquired by an ImmunoSpot S5 Analyser of an NK‐TVA assay where ethanol, ZIKV NS1 or IgG Ab was added to the wells containing effector and target cells. (c) Using an optimized number of target cells, effector PBMC as a source of NK cells and different dilutions of naïve sera ( n = 5), positive control sera that opsonized ZIKV NS1 CEM‐NK R targets ( n = 5) and experimental sera ( n = 12), the loss of target cells at the single‐cell level was measured by the NK‐TVA image cytometry assay and calculated as a percentage of lysed target cells. * P < 0·05 and ** P < 0·01 were obtained using a Kruskal–Wallis test with Dunn's multiple comparisons test

Article Snippet: We generated CEM‐NK R cells stably expressing ZIKV NS1 and established assays using a ZIKV NS1‐specific human IgG1 mAb (Native Antigen Company).

Techniques: Lysis, Cytometry, Positive Control

Operation of the MOnITOR chip for one-step indirect immunoassays. (a) In the ‘Preparation’ stage, the chip immediately started to operate. (b) Engineered bead lines were secured on the reaction zone. The scale bar represents 100 μm. (c) Red-dyed sample was loaded. Trapped gas bubbles eventually diffused through the PDMS matrix (blue arrows). Wasted volumes in the loading and dummy channels consume only 1.6% of the loaded sample. (d,e) Sequentially-loaded blue-dyed buffer flowed and filled the storage chamber. During storage chamber filling, 2nd Ab was rehydrated. Rehydrated 2nd Ab diffusion toward the buffer inlet was prevented by the delaying channel. (f) When loading sample with anti-ZIKV NS1 and anti-CHIKV E2 mAb, only the corresponding beads reacted. (g) Buffer continuously flowed toward the reaction zone by contouring the empty sample inlet. (h) Unbound 2nd Ab were washed by flowing buffer. An image of the reaction zone was acquired in this stage. The scale bar represents 1 cm. (i) In the case of (f), the transferred 2nd Ab only reacted with ZIKV, CHIKV, and + beads. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Biosensors & Bioelectronics

Article Title: A single snapshot multiplex immunoassay platform utilizing dense test lines based on engineered beads

doi: 10.1016/j.bios.2021.113388

Figure Lengend Snippet: Operation of the MOnITOR chip for one-step indirect immunoassays. (a) In the ‘Preparation’ stage, the chip immediately started to operate. (b) Engineered bead lines were secured on the reaction zone. The scale bar represents 100 μm. (c) Red-dyed sample was loaded. Trapped gas bubbles eventually diffused through the PDMS matrix (blue arrows). Wasted volumes in the loading and dummy channels consume only 1.6% of the loaded sample. (d,e) Sequentially-loaded blue-dyed buffer flowed and filled the storage chamber. During storage chamber filling, 2nd Ab was rehydrated. Rehydrated 2nd Ab diffusion toward the buffer inlet was prevented by the delaying channel. (f) When loading sample with anti-ZIKV NS1 and anti-CHIKV E2 mAb, only the corresponding beads reacted. (g) Buffer continuously flowed toward the reaction zone by contouring the empty sample inlet. (h) Unbound 2nd Ab were washed by flowing buffer. An image of the reaction zone was acquired in this stage. The scale bar represents 1 cm. (i) In the case of (f), the transferred 2nd Ab only reacted with ZIKV, CHIKV, and + beads. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Anti-ZIKV NS1, anti-DENV NS1, anti-CHIKV E2, and anti-Mayaro virus (MAYV) E2 monoclonal antibodies (mAb) were also provided from BioNano Health Guard Research Center.

Techniques: Diffusion-based Assay